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. 2023 Feb 23;6:210. doi: 10.1038/s42003-023-04573-z

Fig. 3. Downregulation of LPLA2 levels results in a decrease in BMP levels in HeLa cells.

Fig. 3

a Left panel, representative maximum intensity projections of HeLa cells treated with a control non-targeting siRNA or with an siRNA targeting LPLA2, immunostained for endogenous LPLA2 (magenta) and BMP (green). Scale bar, 5 μm. Right panel, quantification of integrated densities of LPLA2 and BMP fluorescence signals in cells treated with an siRNA targeting LPLA2 (54 ± 3% and 67 ± 3%, respectively, n = 34 cells, three independent experiments) or with a control non targeting siRNA (100 ± 4% and 100 ± 3%, respectively, n = 30 cells, three independent experiments). *** stands for p < 0.001 in Student’s unpaired t-test. b Quantification of overall BMP levels (left) and individual BMP species (right) by LC-MS/MS. Dark blue bars: control siRNA (n = 6 treatments); light blue bars: siRNA directed against LPLA2 (n = 11 treatments). ** stands for p < 0.01 in multiple unpaired t-tests with Welch’s correction. All values are given as mean ± s.e.m.