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. 2022 Oct 25;28(1-2):186–198. doi: 10.1007/s10495-022-01775-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The effect of LACTB induction under 3D and in vivo conditions. (A) Number of spheres in control MCF7ras cells and MCF7ras where LACTB was induced for 6 days. Average ± SD of three experiments are plotted in the graph. (B) FACS analysis of Annexin V staining in spheres with and without LACTB induction. Early apoptosis was considered. (C) Western blot analysis of apoptotic pathways in MCF7ras spheres after LACTB induction for the indicated time points. (D and E) Immunofluorescence analysis of tissue sections of control (MCF7ras -LACTB) and cells overexpressing LACTB (MCF7ras + LACTB) after one week of treatment with DOX Samples were stained with Ki-67 and BAX (E) to examine the proliferation and cell death, respectively. Scale bar: 50 μm. *P < 0.05; **P < 0,01 vs. cells not expressing LACTB.