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. 2023 Feb 23;14:1041. doi: 10.1038/s41467-023-36683-x

Fig. 4. AD-6 antibodies recognise gB vaccine, recombinant gB and infected cells but not virions.

Fig. 4

ad An anti-AD-6 polyclonal antibody was assayed by ELISA for binding to AD-6 polypeptide (a), vaccine gB (b), a commercial recombinant gB produced in E. coli (c) or CMV virions (d). Antibody levels are represented in function of absorbance (450 nm) per concentration of primary antibody in μg/mL and is representative of three independent experiments. Each data point represents mean + SEM of antibodies purified from two immunized rabbits. e Flow cytometry analysis of mock-infected and CMV-infected cells with anti-AD-6 pAb or non-specific rabbit IgG 3 days post infection. Gated on single, live cells and HLA class I expression to identify virally infected cells.