Skip to main content
. 2023 Feb 23;14:1041. doi: 10.1038/s41467-023-36683-x

Fig. 7. The AD-6 polypeptide reverses the control of HCMV spread by human sera taken from gB vaccine recipients.

Fig. 7

a–c To measure the spread of cell-associated virus in HFFs, cells were infected with Merlin-IE2-GFP (MOI = 0.01). At 1 dpi and 5 dpi, healthy seronegative sera (control), or sera from placebo (a) or gB vaccinated (b, c) individuals was added at a 1:20 dilution. Additionally, sera were also pre-incubated with AD-6 peptide prior to addition to the infected cells (+AD-6). 10 dpi, cells were then analysed by IF for viral spread. The percentage of infected cells was measured by anti-IE stain counterstained with nuclei stain and counted by automated fluorescence microscopy (n = 3 where each point is the average of a biological duplicate that was performed for three independent experiments). P values were calculated by a two-sided Mann-U-Whitney comparison of means test. *p = 0.00512; where a p value is not indicated differences were non-significant. The error bars represent 1 standard deviation from the mean.