Fig. 6. USP8 deficiency combined with αPD-L1 promotes antitumor immunity.

a Schematic of the protocol of USP8 deficiency and αPD-L1 combination therapy for orthotopic KPC parental and Usp8 KD cell (5 × 10⁵)-bearing mice. b Photographs of tumors removed from the mice of each group (n = 5). c The statistical plot of tumor weights of the four groups (n = 5). d Flow cytometry of PD-L1 levels in an orthotopic tumor model, statistical results (e) are shown. f Flow cytometry of MHC-1 levels in an orthotopic tumor model, statistical results (g) are shown. h, i Flow cytometry of CD3 + T cells, CD8 + T cells, IFN-γ + CD8 + T cells, TNF-α + CD8 + T cells in the tumor region and the statistical analysis of the results (n = 5). j, k Representative images of IHC staining and quantification of TILs (n = 5). Scale bars = 250 μm. The results are displayed as the means ± SD from representative experiments in (c, e, g, i and k). The data represent three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 assessed via a two-tailed t test; ns not significant.