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. 2022 Dec 16;30(2):515–526. doi: 10.1038/s41418-022-01101-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Hematoxylin and eosin (H&E) staining of A male and B female control and ΔNp63MECcKO submandibular glands 2 months post ΔNp63 deletion. C Saliva volume of the control and ΔNp63MECcKO mice 2 months post TAM administration. Immunochemical analysis of D male and E female control and ΔNp63MECcKO salivary glands show that the SMA⁺/ΔNp63⁺ and SMA⁺/K14⁺ double positive myoepithelial cell subpopulation decreases upon deletion of ΔNp63 in the MECs. Deletion of ΔNp63 in the myoepithelial cells does not appear to affect the other cell lineages as the acinar and ductal markers remain unchanged. White arrows indicate SMA⁺/ΔNp63⁺ MECs or ΔNp63⁺/K14⁺ basal cells. Yellow arrows depict background staining. Myoepithelial and basal cell marker: SMA, Cnn1, ΔNp63, K14; acinar cell marker: Aqp5, Prol1; ductal cell marker; K7, Sox9. Scale bar: 50 µm. (n = 4). F Quantification of SMA and ΔNp63 positive cells upon targeted deletion of ΔNp63. (n = 3). G Quantification of SMA and K14 positive cells upon targeted deletion of ΔNp63. Data are represented as mean ± standard deviation (SD) (n = 3). *p < 0.05, **p < 0.01. White arrows indicate p63⁺ cells, yellow arrows indicate non-specific p63 staining. Immunofluorescence staining colors: Yellow-green and red co-localization, Pink-red and blue (nuclei) co-localization, Turquoise-green and blue co-localization.