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. 2022 Dec 16;30(2):515–526. doi: 10.1038/s41418-022-01101-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Experimental timeline used to collect the regenerated SMGs of the control and ΔNp63MECcKORFP mice. B Histological analysis of the regenerated female control and ΔNp63MECcKORFP glands. C Immunostaining images of regenerated KO glands stained with the MEC markers SMA and ΔNp63. D Immunochemical analysis shows that RFP co-localizes with K7, Sox9 (ductal marker), and Mist1 (acinar marker) in both the control and ΔNp63MECcKORFP regenerated SMGs. RFP⁺ cells represent the progeny of the SMA⁺ myoepithelial cells. E RFP⁺ cells co-localize with Aqp5 and Nkcc1 (acinar markers) in the control and ΔNp63MECcKORFP-regenerated SMGs. White arrows indicate co-localization of RFP and specific cell population markers. Scale bar: 50 µm.