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. 2022 Dec 17;30(2):407–416. doi: 10.1038/s41418-022-01092-y

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Fig. 3 — A Representative flow cytometric analysis of the kinetics over 5 days of ChAT-GFP expression in naïve CD4⁺ T cells that were polarized towards the Th17 cell fate through stimulation with plate-bound anti-CD3 plus soluble anti-CD28 in the presence of PM. B Representative flow cytometric analysis of ChAT-GFP expression by CD4⁺ T cells among total splenocytes that were isolated from mice of the indicated genotypes and cultured for 3 days in the presence of PM plus either MOG or NFM peptide (0.4 mM). C Quantitation of the data shown in B. Each data point represents a technical replicate, with a total of 4 biological replicates, from a combination of two independent experiments. Data in A and B are representative of two independent experiments.