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. 2023 Feb 23;6:209. doi: 10.1038/s42003-023-04574-y

Table 2.

CSM values at λex = 280 nm determined by REES fluorescence spectroscopy and Stern–Volmer constants (ksv) derived from acrylamide quenching experiments.

Protein CSM280 at 25 °C CSM280 at 37 °C ksv at 25 °C
nm nm L/mol
P-CL 351.00 351.93 3.39
G-CL 344.47 344.65 1.33
P-LC 348.68 351.02 3.27
G-LC 345.16 345.02 1.28
P-LC G136V 348.32 348.79 2.32
G-LC V136G 348.67 349.71 3.28

All experiments were carried out as duplicates (n = 2) and the mean values of the two experiments are reported.