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. 2023 Feb 23;13:3191. doi: 10.1038/s41598-023-30294-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Waterfall-plots illustrating the distribution of RNF43 (a) and LRP1B (b) immunostaining among 446 patients. The waterfall plots show case-by-case the results of immunohistochemistry (RNF43-IHC and LRP1B-IHC; linear data presentation). The different colours illustrate the different staining intensities: RNF43-IHC 0 (blue), RNF43-IHC 1+ (light green) and RNF43-IHC 2+ (medium green) as well as LRP1B-IHC 0 (blue), LRP1B-IHC 1+ (light green), LRP1B-IHC 2+ (medium green) and LRP1B-IHC 3+ (dark green). The sum total of all staining intensities found in a single case always added to a total H-score of 100% according to the following formulas: P (RNF43-IHC 0) + P (RNF43-IHC 1+) + P (RNF43-IHC 2+) = 100% and P (LRP1B-IHC 0) + P (LRP1B-IHC 1 +) + P (LRP1B-IHC 2+) + P (LRP1B-IHC 3+) = 100%.