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. 2022 Nov 17;30(2):383–396. doi: 10.1038/s41418-022-01090-0

Fig. 5. ARID2 activates the transcription of NEDD4L by recruiting CARM1.

Fig. 5

A The influence of ARID2 on the promoter activity of NEDD4L was examined by luciferase reporter assay. B Silver-stained gel showing differential bands between control and ARID2-overexpressing samples. C The interaction between ARID2 and CARM1 was examined by Co-IP assay (left panel) and GST pull-down assay (right panel) in 293T cells co-transfected with the indicated plasmids. Purified GST was used as a control. D Co-IP assay conducted in primary hepatocytes verified the endogenous interaction between ARID2 and CARM1. IgG was used as a negative control. E The interactions between exogenous CARM1 and truncated ARID2 mutants were examined in 293T cells by Co-IP. F The lipid droplets in control and ARID2-overexpressing Hep3B cells with reduced expression of CARM1 were examined by Nile Red staining. Scale bars, 90 μm. Blue signal, nuclei stained with Hoechst; Red signal, lipid droplets stained with Nile Red. G, H Protein and mRNA level of NEDD4L were measured in control and ARID2-overexpressing Hep3B cells with CARM1 knockdown. All experiments were repeated 3 times independently. Relative promoter activity of NEDD4L in panel A was analyzed by two-tailed unpaired Student’s t test, other data were analyzed with one-way ANOVA with Bonferroni post hoc multiple comparison test. All results were represented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.