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. 2023 Feb 23;13:3138. doi: 10.1038/s41598-023-29868-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CB1R immunostaining of hippocampus. Representative microphotographs of CB1R immunoreactivity in the hippocampus of a control dog (a–d), a dog with structural epilepsy (e) and a dog with idiopathic epilepsy (f). Pyramidal cell layer (p.c.l.) exhibits strong CB1R signal surrounding the unstained soma (a, b) with intense dot-like CB1R immunoreactivity (arrow in b). The dentate gyrus of the hippocampus demonstrates distinct dot-like CB1R immunoreactivity in the molecular layer (m.l.) and hilus (h), whereas granule cell layer (g.c.l.) appears to be devoid of CB1R immunoreactivity (a, d, e, f). The circle shows strong CB1R labeling of cells with astrocytic morphology (c): p.l.- polymorphic layer; s.o.—stratum oriens, s.r.—stratum radiatum.