FIG. 3.

Regulation of mannoprotein gene expression during cold shock. Cells of strain FY23 were subjected to cold shock as described in Materials and Methods and harvested for RNA extraction. (A) Northern blots were probed with TIP1, TIR1, and TIR4. (B) Cells of strain FY23 and FY23mox4Δ were grown under aerobic conditions, under anaerobic conditions, or at 13°C. A Northern blot was probed with CWP1. (C) The same blot was probed with TIR4. (D) Cells subjected to cold shock at different temperatures were harvested for RNA extraction. Northern blots were probed with TIP1 and CWP1.