FIG. 6.

Regulatory factors controlling expression of CWP1 and CWP2. (A) Cells of strains FY23, FY23mot3Δ, and FY23tup1Δssn6Δ were grown in YPD under aerobic and anaerobic conditions and harvested for RNA extraction. Northern blots were probed with CWP1, CWP2, and ACT1. Phosphorimager data (in arbitrary units) for relative intensities are shown at the bottom. (B) Cells of strains FY23 and FY23mox4Δ were grown under anaerobic conditions in YPD. Phosphorimager data are shown. (C) Cells of strain FY23 carrying YCpGAL1/MOX4 or the YCplac33 vector were grown at 30°C for 4 h under aerobic conditions in SC-galactose-raffinose medium (1) and harvested for RNA extraction. Data from an autoradiographic scan are shown.