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. 2023 Feb 10;14:1095098. doi: 10.3389/fimmu.2023.1095098

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Copyright © 2023 Zhang, Zhang, Zhang, Song, Bian, Yi and Ma

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exploration of neutrophil-associated subtypes and biological features. (A) Consensus matrix heatmap defining two clusters (k = 2) and their correlation area. (B) PCA analysis showed significant differences in transcriptomes between the two subtypes. (C, D) Heatmap and boxplot of the expression of 24 NADEGs in different clusters. Red for upper regulation, blue for lower regulation. (E) GSVA of biological pathways between two distinct subtypes, in which red represented activated and blue inhibited pathways. (F) The ssGESA results indicated the abundance of 28 infiltrating immune cells in different subtypes. The asterisks represented the statistical P-value (*P < 0.05; **P < 0.01; ***P < 0.001). (G) Correlations between the two subtypes and TME score. The asterisks represented the statistical P-value (*P < 0.05; **P < 0.01; ***P < 0.001).