Fig. 2.

Nox4 deficient primary mouse skin fibroblasts were able to differentiate into myofibroblasts after TGF-β2 stimulation. A. Nox4 mRNA expression in WT primary skin fibroblasts cultured without (Control) or with TGF-β2; B. Acta2 mRNA expression in WT and Nox4 KO primary skin fibroblasts cultured without or with TGF-β2; C. Percentage of α-SMA-positive cells in WT and Nox4 KO primary skin fibroblasts cultured without or with TGF-β2; D. Nox4 mRNA expression in WT and p22^phox KO primary skin fibroblasts cultured without or with TGF-β2; E. Acta2 mRNA expression in WT and p22^phox KO primary skin fibroblasts cultured without or with TGF-β2; F. Percentage of α-SMA-positive cells of WT and p22^phox KO primary skin fibroblasts cultured without or with TGF-β2; G. Representative immunofluorescence staining of α-SMA (green) and DAPI (blue) in WT, Nox4 KO and p22^phox KO primary skin fibroblasts cultured without or with TGF-β2; H. Representative Western blot for α-SMA in WT, Nox4 KO; I. Quantification of α-SMA band intensity from primary skin fibroblasts cultured without or with TGF-β2 in WT and Nox4 KO primary skin fibroblasts, GADPH was used as loading control; WT (green bars) n = 3, Nox4 KO (red bars) n = 3; Data shown as mean ± SD; ns – not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; Two-way ANOVA. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)