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Phenotype identification of colorectal cancer stem cells (CCSCs). (a) Phenotypes of suspension stem cells. Count and FITC-A are axis labels, #1 is gate label, and there were no quartile labels. (b, c) Western blotting and quantitative analysis of stemness markers, including SOX2, OCT4, and Nanog, in CCSCs. (d) qRT-PCR analysis of stemness markers, including SOX2, OCT4, and Nanog, in CCSCs. Data are presented as mean ± SD; ∗∗P < 0.01.
