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. 2023 Feb 16;2023:4772134. doi: 10.1155/2023/4772134

Figure 3.

Figure 3

VD-induced ferroptosis in CCSCs. (a) Levels of ROS in the CCSCs treated with different concentrations of VD, as detected using flow cytometry. Results are expressed as percentages; bar graph summarises quantitative data representing the mean ± SD of values from three independent experiments. (b) Levels of Cys in the CCSCs treated with different concentrations of VD. (c) Levels of GSH in the CCSCs treated with different concentrations of VD. (d) Transmission electron microscopy of CCSCs treated with different concentrations of VD. The images below show an enlarged version of the red area. The red arrows indicate the thickening of the mitochondrial membrane and the narrowing of the mitochondria. The yellow arrows indicate the mitochondrial crista. The blue arrow indicates the ruptured mitochondria. Scale bars of the picture above: 5 μm; scale bars of the picture below: 1 μm. (e, f) Western blotting and quantitative analysis of GPX4, SLC7A11, and COX2 in CCSCs treated with different VD concentrations. (g) qRT-PCR analysis of GPX4, SLC7A11, and COX2 in CCSCs treated with different concentrations of VD. Data are presented as mean ± SD; ∗P < 0.05 and ∗∗P < 0.01.