Table 3.

Assay characteristics for all cytokines of MILLIPLEX^® bovine cytokine immunology multiplex assay (Cat. # BCYT1-33K).

Cytokine	Accuracy	Sensitivity	Standard Curve Range	Intra-assay %CV	Inter- assay %CV
IL-1α	94%	0.36	0.3 - 5,000 pg/ml	<10%	<10%
IL-1β	91%	4.93	2.6 - 40,000 pg/ml	<10%	<10%
IL-6	95%	11.23	2.6 - 40,000 pg/ml	<10%	<15%
IL-8	95%	22.6	2.2 - 35,000 pg/ml	<10%	<10%
IL-17A	103%	0.67	0.6 - 10,000 pg/ml	<10%	<10%
IFN-γ	94%	0.08	0.1 - 2,000 pg/ml	<10%	<10%
TNF-α	92%	22.62	12.8 - 200,000 pg/ml	<10%	<15%
IL-4	93%	16.57	12.8 - 200,000 pg/ml	<10%	<10%
IL-10	96%	1.05	0.96 - 15,000 pg/ml	<10%	<10%

IL, Interleukin; IFN-γ, Interferon gamma; TNF-α, Tumor necrosis factor.