FIG. 1.

Physical map of the B. bronchiseptica bupI bupR bfrZ locus and relevant constructions described in this study. The recombinant plasmid-associated Lac phenotypes in the FURTA are indicated on the right. In this genetic test, a Lac⁺ phenotype indicates the presence of an FBS on a multicopy plasmid. The boxes representing ORFs are drawn to scale; the arrowheads indicate the direction of transcription. The TnphoA insertion site is indicated by an arrow. Certain restriction sites are indicated as follows: B, BamHI; H, HincII; K, KpnI; N, NsiI; P, PstI; RV, EcoRV; S, SalI; SI, SacI; Sc, ScaI; Sm, SmaI; Sp, SphI; St, StuI.