Figure 6.

Tivantinib prevents the development of EAE

(A–E) Intraperitoneal injection of vehicle or Tivantinib (10 mg/kg) in C57BL/6J mice beginning at the induction of EAE every 2 days. (A) Clinical scores after EAE induction. (B) Sections of paraffin-embedded spinal cord tissues were stained with H&E and Luxol fast blue (LFB). (C) FACS analysis of live CD4⁺, CD8⁺, CD11b⁺, and CD11b⁻ cells gate on CD45^hi cells in the CNS on day 22 after EAE induction. (D) The numbers of live CD4⁺, CD8⁺, CD11b⁺, and CD11b⁻ cells gate on CD45^hi cells in the CNS on the 22 days after the induction of EAE. (E) The mRNA levels of IL-1β in CNS were evaluated by real-time PCR.

(F) Western blot analysis of NLRP3, ASC, and caspase-1 in the spinal cord of EAE mice. Data represent means ± SEM from five biological duplicates. Statistical analysis was performed using one-way ANOVA(A, D) or unpaired Student’s t test (E). ∗∗p < 0.01, ∗∗∗p < 0.001.