REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mouse IL-1β	R&D Systems	Cat#AF-401-NA; AB_416684
anti-NLRP3	Adipogen	Cat#AG-20B-0014; AB_2885199
anti-mouse caspase-1 (p20)	Adipogen	Cat#AG-20B-0042; AB_2490248
Anti-β-actin	Abmart	Cat#P30002; AB_2222847
Anti-human IL-1β	Proteintech	Cat#60136-1-Ig; AB_10597543
Anti-human caspase-1	Cell Signaling	Cat#2225; AB_2243894
Anti-ASC	Santa Cruz	Cat#sc-22514-R; AB_2174874
anti-NEK7	Santa Cruz	Cat#SC-50756; AB_2235871
Anti-Flag	Sigma	Cat#F2555; AB_796202
anti-VSV	Sigma	Cat#V4888; AB_261872
Chemicals, peptides, and recombinant proteins
Tivantinib	TargetMol	Cat#T6117
NVP-BVU972	TargetMol	Cat#T2680
AMG-337	TargetMol	Cat#T3209
SGX-523	TargetMol	Cat#T2293
PMA (phorbol-12-myristate-13-acetate)	Sigma	Cat# P8139
Poly (dA:dT)	Sigma	Cat#86828-69-5
Nigericin	Sigma	Cat# N7143
ATP	Sigma	Cat#34369-07-8
MSU	Sigma	Cat#69-93-2
protein G agarose	Sigma	Cat# P7700
Pam3CSK4	Invitrogen	Cat#112208-00-1
ultrapure LPS	Invitrogen	Cat#297-473-0
MitoSOX	Invitrogen	Cat#M36008
Lipofectamine 2000	Invitrogen	Cat#11668019
DAPI	Invitrogen	Cat# D21490
MQAE	Invitrogen	Cat#162558-52-3
Critical commercial assays
LDH Cytotoxicity Assay Kit	Beyotime	Cat#C0017
ADP-Glo Kinase Assay Kit	Promega	Cat#V6930
Experimental models: Cell lines
HEK293T	ATCC	ATCC CRL-3216
THP-1	ATCC	ATCC TIB-202
Experimental models: Organisms/strains
C57BL/6J	Jackson Laboratory	000664
Oligonucleotides
Mouse IL-1β forward	Sangon	5′TGCCACCTTTTGACAGTGATG-3′
Mouse IL-1β reverse	Sangon	5′AAGGTCCACGGGAAAGACAC-3′
Mouse Gapdh forward	Sangon	5′GGTGAAGGTCGGTGTGAACG-3′
Mouse Gapdh reverse	Sangon	5′CTCGCTCCTGGAAGATGGTG-3′
Software and algorithms
ImageJ (v1.51)	ImageJ Software	ImageJ (nih.gov)
GraphPad Prism 8.0	GraphPad Software	Prism - GraphPad
FlowJo software (v10.0.7) Article	FlowJo software	Download FlowJo | FlowJo, LLC