FIG. 1.

Cloning strategy and restriction map of the B. lactis xfp gene and its adjacent region. (A) Amino acids (7 to 28) from the N terminus of the Xfp protein, position of deduced primers (arrows), and corresponding nucleotide sequence. (B) Open reading frames guaA, xfp, and pta (open and filled arrows) and restriction sites which were relevant for cloning. (C) Inserts of recombinant plasmids (solid lines) bordered by dashed vertical lines representing either restriction sites or 5′ ends of primers used for PCR; primers are indicated by short arrows and described in detail in Table 1. Numbers at the end of vertical dashed lines are assigned to nucleotide numbers as indicated in the text and in the GenBank database.