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Purification of Xfp from B. lactis
| Step | Vol (ml) | Total units | Total protein (mg) | Sp act (Ua/mg) | Purification factor | Yield (%) |
|---|---|---|---|---|---|---|
| Crude extract | 15 | 93.4 | 246.0 | 0.38 | 1.0 | 100 |
| DEAE-cellulose | 138 | 28.7 | 47.1 | 0.61 | 1.6 | 30.7 |
| Mono Q | 2.1 | 15.5 | 7.4 | 2.10 | 5.5 | 16.6 |
| Superdex 200 | 6.0 | 4.1 | 0.96 | 4.28 | 11.1 | 4.4 |
One unit is defined as the amount of extract forming 1 μmol of acetyl phosphate per min with F6P as a substrate.
