TABLE 1.
Biochemical and biophysical properties of VHH‐based NKCEs targeting NKp46 and EGFR.
| # | Yield (mg/L) | SEC (%) | KD (M) | k on (1/Ms) | k off (1/s) |
|---|---|---|---|---|---|
| NKp46.1 SEEDbody eff− | 190 | 94.6 | 3.84E−09 | 6.07E+05 | 2.33E−03 |
| NKp46.2 SEEDbody eff− | 176; 176 a | 94.4; 92.1 a | 1.05E−09 | 2.85E+05 | 2.97E−04 |
| NKp46.3 SEEDbody eff− | 147 | 96.1 | 1.71E−09 | 3.06E+05 | 5.24E−04 |
| NKp46.4 SEEDbody eff− | 180 | 94.7 | 1.20E−09 | 6.32E+05 | 7.55E−04 |
| NKp46.5 SEEDbody eff− | 234 | 96.3 | 5.01E−10 | 6.48E+05 | 3.25E−04 |
| NKp46.6 SEEDbody eff− | 228 | 94.8 | 4.42E−09 | 1.05E+05 | 4.62E−04 |
| NKp46.7 SEEDbody eff− | 200 | 92.9 | 6.89E−09 | 1.48E+05 | 1.02E−03 |
| NKp46.8 SEEDbody eff− | 175 | 91 | 1.34E−09 | 5.89E+05 | 7.90E−04 |
| NKp46.9 SEEDbody eff− | 166 | 93.3 | 1.43E−09 | 6.85E+05 | 9.77E−04 |
| NKp46.10 SEEDbody eff− | 168 | 96.8 | 2.65E−10 | 9.41E+04 | 2.50E−05 |
| NKp46.11 SEEDbody eff− | 172 | 97.9 | 2.31E−09 | 2.63E+05 | 6.07E−04 |
| NKp46.12 SEEDbody eff− | 165 | 94.9 | 4.09E−09 | 5.56E+05 | 2.27E−03 |
| NKp46.13 SEEDbody eff− | 181 | 87.1 | 1.87E−09 | 5.78E+05 | 1.08E−03 |
| NKp46.14 SEEDbody eff− | 183 | 96.7 | 1.94E−09 | 2.90E+05 | 5.63E−04 |
| NKp46.15 SEEDbody eff− | 171 | 96.3 | 5.34E−09 | 2.96E+05 | 1.58E−03 |
| NKp46.16 SEEDbody eff− | 170 | 93.4 | 1.05E−08 | 3.04E+05 | 3.19E−03 |
| NKp46.17 SEEDbody eff− | 141 | 96.7 | 1.85E−08 | 6.25E+04 | 1.16E−03 |
| NKp46.18 SEEDbody eff− | 177 | 93.6 | 6.85E−09 | 2.44E+05 | 1.67E−03 |
| NKp46.20 SEEDbody eff− | 131 | 83.9 | 5.50E−09 | 1.12E+05 | 6.16E−04 |
| NKp46.21 SEEDbody eff− | 92 | 95.7 | 1.94E−09 | 7.29E+05 | 1.41E−03 |
| NKp46.22 SEEDbody eff− | 164 | 95.5 | 5.34E−09 | 2.70E+05 | 1.44E−03 |
| NKp46.23 SEEDbody eff− | 80 | 98.5 | 2.20E−09 | 1.06E+05 | 2.34E−04 |
| NKp46.24 SEEDbody eff− | 19 | 95.3 | 5.58E−09 | 9.57E+04 | 5.34E−04 |
| NKp46.25 SEEDbody eff− | 72 | 95 | 2.12E−09 | 3.43E+05 | 0.000728 |
| NKp46.26 SEEDbody eff− | 146; 152 a | 95.4; 86.9 a | 1.46E−08 | 3.27E+05 | 4.80E−03 |
| NKp46.27 SEEDbody eff− | 198 | 95.5 | 6.85E−09 | 2.54E+05 | 1.74E−03 |
| NKp46.28 SEEDbody eff− | 102 | 94.7 | 2.85E−09 | 1.68E+05 | 4.78E−04 |
| NKp46.29 SEEDbody eff− | 150 | 81.8 | <1.0E−12 | 1.05E+05 | <1.0E−07 |
| NKp46.31 SEEDbody eff− | 168 | 94.4 | 2.55E−08 | 3.94E+04 | 1.00E−03 |
| NKp46.34 SEEDbody eff− | 121 | 96.3 | 1.93E−09 | 2.93E+05 | 5.66E−04 |
Note: Nonbinding molecules were excluded from this table. k on is the rate constant of association, while k off is the rate constant of dissociation.
a
Indicate the values for a second expression of the respective molecules.