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SDS-PAGE and Western blot analysis of the rpoN, fliA, and flgR mutants compared to the wild-type C. jejuni NCTC 11168. In panel A, lane 1 contains a broad-range protein marker, and in panel B, lane 1 contains a prestained broad-range protein marker. In both panels A and B, lane 2 shows the ς54 mutant, lane 3 shows the ς28 mutant, lane 4 shows the FlgR mutant, and lane 5 shows wild-type strain NCTC 11168. Arrows show the location of the flagellin band.
