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. 2023 Jan 10;10(6):2204006. doi: 10.1002/advs.202204006

Figure 2.

Figure 2

5‐HT mediated ferroptosis resistance is independent of canonical anti‐ferroptosis pathway. A) Western blot analysis of 5‐HT receptors in a panel of cancer cell lines. B) HT1080 cells expressing shRNA of 5‐HT receptors were treated with RSL3 (200 nm) and 5‐HT (10 µm). Cell viability was assessed 24 h thereafter using CCK8. C) Western blot analysis of arrestin β1 and arrestin β2 in 293T arrestin β1‐/‐, arrestin β2‐/‐ DKO cells. D) Cell death measurement of 293T arrestin β1‐/‐, arrestin β2‐/‐ DKO cells treated with RSL3 (2.5 µm) and Fer‐1 (1 µm) for 8 h. E) Western blot analysis of p‐mTOR and p‐AMPK in a panel of cell lines upon 5‐HT treatment (10 µm) for 12 h. F) RNA‐seq analysis of HT1080 cells with or without 5‐HT treatment (10 µm) for 12 h. G) Volcano plots showing the alteration of phospholipid components in HT1080 cells treated with 5‐HT (10 µm) for 12 h.