Fig. 3.

Inhibition of IL-17 alleviates Aβ₄₂-induced cognitive deficits. Flow chart for the experiments (A). IL-17 levels were detected by western blotting using specific antibodies, and actin was used as a loading control (B). Intensity analysis of IL-17 levels (C). N = 3. ELISA to measure the levels of IL-17 (D), N = 3. The open-field test showed the total distance covered (E) and the time of center duration (F) of the three groups. NORT showed the time spent exploring new objects at 2 h (G) and 24 h (H). The contextual fear conditioning test determined the freezing time at 2 h (I) and 24 h (J). N = 10 for independent experiments. Data are presented as the mean ± SD. p value significance is calculated from a one-way ANOVA test, *p < 0.05, **p < 0.01 and ***p < 0.001 vs the Aβ₄₂ group. For western blotting original images, please see Additional file 1, and Adobe Photoshop software (2021) was used to cut the parts of interest from the original images