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. 2023 Feb 10;14:1100161. doi: 10.3389/fimmu.2023.1100161

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Copyright © 2023 Humphries, O’Connor, Stewart, Quinn, Gaughan, Mills, Williams, Stone, Finlayson, Chabaud-Riou, Boudet, Dhaliwal and Pavot

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In Vitro Human T_RM and B_RM Detection using KronoScan and Panoptes. (A) Cells from human lung tissue digests were stained with T_RM or B_RM antibodies and imaged in vitro using KronoScan imaging system and Panoptes multifunctional fibre. (B) Human T_RM detection. T_RM were detected via expression of CD69 and CD103. Blue arrow indicates CD69⁺ cells and yellow arrow points CD69⁺CD103⁺ T_RM. (C) Human B_RM detection. Blue arrow indicates CD69⁺ cells and yellow arrow points CD69⁺ CD20⁺ B_RM. Representative images from n=3 experiments.