Figure 5.

In Situ Detection of Pre-labelled T_RM/B_RM in Human Lung. (A) Pre-labelled human pulmonary T_RM and B_RM in suspension were instilled into the alveolar space and detected using KronoScan and Panoptes during human EVLV. (B) Baseline lung imaging prior to pre-labelled cell instillation. (C) In situ imaging of pre-labelled CD69⁺ cells. Lung tissue digest cells were stained with fluorescent CD69 and CD103 antibodies prior to instillation into the alveolar space using Panoptes. Cells were imaged with KronoScan. Blue arrow indicates CD69⁺ cells. (D) In situ imaging of pre-labelled CD69⁺CD103⁺ T_RM. Yellow arrow indicates CD69⁺CD103⁺ T_RM. (E) In situ imaging of pre-labelled B_RM. Lung tissue digest cells were stained with fluorescent CD20 and CD69 antibodies prior to instillation to the alveolar space. Blue arrow indicates CD69⁺ cells and yellow arrow points CD69⁺CD20⁺ B_RM. Representative images from n=3 experiments.