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Research flowchart. In the first step, in a sample size of six dogs, a subset of miRNAs is identified by NGS as stable miRNAs. The white box shows all the phases performed in the first step: blood sampling at T0 and T1, serum RNA extraction, NGS circulating miRNA profiling, and stable miRNA selection. In the second step (gray boxes), the phases are stable miRNA qPCR validation (22 dogs) and bioinformatics analysis of qPCR output data to determine EC miRNAs’ stability ranking.
