Table 2.
QPCR-specific pair primers. The resuspended primer mix contains both forward and reverse sequences. * Previous studies have identified miR-320 as the best EC miRNA in SAR dog serum.
| Primer | Recommended for: | GeneGlobe ID |
|---|---|---|
| UniSp2 | Spike-in (RNA isolation efficiency evaluation) | YP00203950 |
| UniSp4 | Spike-in (RNA Isolation efficiency evaluation) | YP00203953 |
| UniSp6 | Spike-in (RT and PCR inhibitors evaluation) | YP00203954 |
| miR-320 * | Serum EC miRNAs | YP00206042 |
| miR-486 | Probable serum EC miRNAs | YP02119777 |
| miR-16 | Probable serum EC miRNAs | YP00205702 |
| miR-92a | Probable serum EC miRNAs | YP00204258 |
| miR-191 | Probable serum EC miRNAs | YP00205972 |
| miR-223 | Probable serum EC miRNAs | YP00205120 |
| miR-423a | Probable serum EC miRNAs | YP00205624 |
| let-7b | Probable serum EC miRNAs | YP00204750 |
| miR-25 | Probable serum EC miRNAs | YP00204361 |
| miR-93 | Probable serum EC miRNAs | YP00204715 |