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. 2022 Nov 24;13:1032113. doi: 10.3389/fimmu.2022.1032113

Figure 1.

Figure 1

LCMV-specific T cells from DGKζ KO mice show increased activation after LCMV CL 13 infection. (A) The proportion and (B) absolute number (spleen only) of CD8+Tetramer+ (GP33+ or GP276+) cells and the (C) fraction of KLRG1+ cells of CD8+Tetramer+ cells were quantified in the blood and spleen of WT and DGKζ KO mice infected with LCMV CL 13 on Days 7 and 10 post infection. (D) The fraction of CD8+ T cells expressing IFNγ or CD107a was quantified in spleen cells isolated from LCMV CL13-infected WT and DGKζ KO mice on Days 7 or 10 post infection and restimulated with GP33 peptide or PMA and ionomycin (PI). (E) Virus titers in kidney of LCMV CL13-infected WT and DGKζ KO mice on Days 7 or 10 post infection. Data are represented as mean ± SEM of N=6-8 mice/group pooled from 2 independent experiments. (F) Body weight (N=22-27 mice/group from 4 independent experiments) and (G) survival (N=35-40 mice/group from 7 independent experiments) of WT and DGKζ KO mice infected with LCMV CL13. N.S. = not significant, *p<0.05, **P<0.01, ***p<0.001, ****p<0.0001 by Student t-test (A–F) or Log rank (Mantel Cox) test and Gehan-Breslow-Wilcoxon test (G).