Figure 3.

ERK^SEM mice show increased LCMV-specific CD8⁺ T cell activation and reduced virus titers after LCMV clone 13 infection. (A) Survival and body weight of WT and ERK^SEM mice infected with LCMV CL13. N=10-11 mice/group from 2 independent experiments. (B) The proportion and absolute number (spleen only) of CD8⁺Tetramer⁺ (GP33⁺ or GP276⁺) cells and the (C) fraction of KLRG1⁺ cells of CD8⁺Tetramer⁺ cells were quantified in the blood and spleen of WT and ERK^SEM mice infected with LCMV CL 13 on Days 7 and 10 post infection. (D) The fraction of CD8⁺ T cells expressing IFNγ or CD107a was quantified in spleen cells isolated from LCMV CL13-infected WT and ERK^SEM mice on Days 7 or 10 post infection and restimulated with GP33 peptide or PMA and ionomycin (PI). (E) Virus titers in kidney of LCMV CL13-infected WT and ERK^SEM mice on Days 7 or 10 post infection. Data are represented as mean ± SEM of N=6-8 mice/group pooled from 2 independent experiments. N.S. = not significant, *p<0.05, **P<0.01, ****p<0.0001 by Log rank (Mantel Cox) test and Gehan-Breslow-Wilcoxon test (A) or Student t-test (B–E).