Figure 4.

ERK^SEM LCMV-specific T cells display increased proliferation, survival, and memory phenotype. (A) CD8⁺ T cells from WT-P14 (CD45.1⁺) and ERK^SEM-P14 (CD45.2⁺) mice were mixed at a 1:1 ratio and adoptively transferred into Thy1.1⁺ WT host mice 1 day before infection with LCMV CL13. (B) The fraction of WT and ERK^SEM T cells of all adoptively transferred P14 CD8⁺ T cells was quantified over time in the spleen. Data are plotted as mean of N=3-4 mice/group per time point. One representative of 2 independent experiments is shown. (C) The fraction and odds ratio of splenic WT and ERK^SEM CD8⁺ T cells expressing PD-1, 2B4, LAG3, TIM3, TIGIT, or with a SLEC phenotype (KLRG1⁺CD127), (D) expressing a MPEC or T_CM phenotype or Eomes, or (E) expressing Bim or Bcl-2 were quantified at Day 10 post LCMV CL13 infection. Data from N=6 mice/group pooled from 2 independent experiments are shown. N.S. = not significant, *p<0.05, **P<0.01 by paired Student t-test.