Figure 6.

Short-term inhibition of DGKζ increases LCMV-specific CD8⁺ T cell activation and decreases virus titers in the chronic phase of LCMV CL 13 infection. (A) WT mice were infected with LCMV CL13 and treated with either vehicle or ASP1570 on Days 32, 33, and 34 post infection. (B) The proportion (blood and spleen) and absolute number of CD8⁺Tetramer⁺ (GP33⁺ or GP276⁺) cells and the fraction of (C) KLRG1⁺, PD-1⁺, 2B4⁺, LAG3⁺, TIGIT⁺, TIM3⁺, or (D) PD-1⁺KLRG1⁻, Eomes⁺, PD-1 KLRG1⁺Eomes⁺ cells of CD8⁺Tetramer⁺ cells were quantified in the spleen of vehicle and ASP1570-treated mice on Day 35 post LCMV CL13 infection. (E) The fraction of CD8⁺ T cells expressing IFNγ or CD107a was quantified in spleen cells isolated from vehicle and ASP1570 -treated mice on Day 35 post LCMV CL13 infection and restimulated with GP33 peptide or PMA and ionomycin (PI). (F) Virus titers in kidney of vehicle and ASP1570 -treated mice on Day 35 post LCMV CL13 infection. Data from N=7 mice/group pooled from 2 independent experiments are shown. N.S. = not significant, *p<0.05, **P<0.01 by Student t-test.