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. 2022 Nov 24;13:1032113. doi: 10.3389/fimmu.2022.1032113

Figure 9.

Figure 9

Rapamycin treatment rescues DGKζ KO CD8+ T cells from disappearing at Day 14 post infection. (A) CD8+ T cells from WT-P14 (CD45.1+) and DGKζ KO-P14 (CD45.2+) mice were mixed at a 1:1 ratio and adoptively transferred into Thy1.1+ WT host mice 1 day before infection with LCMV CL13. One group of mice was treated with vehicle and another with rapamycin. (B) The fraction of WT and DGKζ KO CD8+ T cells of all adoptively transferred P14 T cells was quantified at Days 10 and 14 post infection in the spleen. The odds ratio of splenic WT vs. DGKζ KO CD8+ T cells was compared in mice treated with vehicle vs. rapamycin at Day 10 post infection. One representative of 2 independent experiments is shown. (C) The odds ratio of splenic WT and DGKζ KO CD8+ T cells expressing a SLEC phenotype (KLRG1+CD127−), an MPEC or TCM phenotype, Eomes, Bim, or Bcl-2 were quantified at Day 10 post LCMV CL13 infection and compared between vehicle and rapamycin treatment. Data from N=8 mice/group pooled from 2 independent experiments is shown. N.S. = not significant, *p<0.05, or ****p<0.0001 by Student t-test.