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. 2023 Feb 24;24:67. doi: 10.1186/s12859-023-05175-6

Table 2.

Physicochemical and immunological properties of the candidate vaccine

Molecular features Prediction
No. of amino acids 590
Molecular weight (kDa) 62.96
Theoretical pI 4.60
Total no. of negatively charged residues (Asp + Glu) 115
Total no. of positively charged residues (Arg + Lys) 64
Estimated half-life  > 10 h (Escherichia coli, in vivo)
 > 20 h (yeast, in vivo)
30 h (mammalian reticulocytes, in vitro)
Instability index 33.71 (Stable)
Aliphatic index 59.63
Grand average of hydropathicity (GRAVY) − 1.028
Solubility (SOLpro) 0.958393 (Soluble)
Antigenicity (VaxiJen) 0.8833 (Probable ANTIGEN)
Antigenicity (ANTIGENpro) 0.850188 (Probable ANTIGEN)
Allergenicity (AlgPred/AllerTOP) Non-allergen