Table 2.
Physicochemical and immunological properties of the candidate vaccine
| Molecular features | Prediction |
|---|---|
| No. of amino acids | 590 |
| Molecular weight (kDa) | 62.96 |
| Theoretical pI | 4.60 |
| Total no. of negatively charged residues (Asp + Glu) | 115 |
| Total no. of positively charged residues (Arg + Lys) | 64 |
| Estimated half-life | > 10 h (Escherichia coli, in vivo) |
| > 20 h (yeast, in vivo) | |
| 30 h (mammalian reticulocytes, in vitro) | |
| Instability index | 33.71 (Stable) |
| Aliphatic index | 59.63 |
| Grand average of hydropathicity (GRAVY) | − 1.028 |
| Solubility (SOLpro) | 0.958393 (Soluble) |
| Antigenicity (VaxiJen) | 0.8833 (Probable ANTIGEN) |
| Antigenicity (ANTIGENpro) | 0.850188 (Probable ANTIGEN) |
| Allergenicity (AlgPred/AllerTOP) | Non-allergen |