NO nanoparticle and DETA NONOate treatment resulted in altered NO pathway. Effects of NO nanoparticle treatment on plasma concentrations of (A) L-arginine, (B) asymmetric dimethylarginine (ADMA), (C) symmetric dimethylarginine (SDMA), and (D) the ratio of L-arginine to ADMA (AAR). (E) Representative Western blots demonstrate endotheial nitric oxide synthase (eNOS, 140 kDa), neuronal nitric oxide synthase (nNOS, 155 kDa), dimethylarginine dimethylaminohydrolase-1 (DDAH1, 31 kDa), and dimethylarginine dimethylaminohydrolase-2 (DDAH2, 30 kDa) bands. (F) The relative protein abundance of renal cortical eNOS, nNOS, DDAH1, and DDAH2 were calculated. The letters a, b, and c indicate the differences between the groups (p < 0.05, one-way ANOVA); n = 8/group.