Figure 4.
Heatmaps of gene expression changes in pathways for cholesterol biosynthesis and modification, glucose metabolism and cell starvation, and fatty acid biosynthesis and metabolism. (A). RNAseq was used to identify gene expression changes in primary human MSCs following 1 or 2 Gy (0.66 cGy/h) 60Co irradiation. Control cells were cultured under identical conditions for 1 or 2 weeks. GO analysis was performed using DAVID and heatmaps were generated using genes with q < 0.05. Rows are centered and unit variance is applied to rows. (B). qPCR gene regulation using log base 2 scale fold change of genes supporting heatmaps of pathways. Data show averages ± SEM N = 3 biological replicates with two technical repeats; * indicates p < 0.05, *** indicates p < 0.001, **** indicates p < 0.0001, respectively, compared with sham irradiated control cells.