Figure 5.
Mitochondrial respiration. (A) Representative records of mitochondrial oxygen consumption measured by high resolution respirometry. (B) Complex I-linked (NADH-generating substrates: glutamate and malate-dependent) respiration, (C) Oxidative phosphorylation (ADP-dependent respiration) in the presence of saturating levels of substrates. (D) Complex I and II-linked (added succinate respiration). (E) Cytochrome c release assessed by adding exogenous cytochrome c to the sample in the presence of glutamate and malate or succinate. (F) Respiratory control ratio (RCR) expressed as a ratio of ADP-dependent oxidative phosphorylation and ADP-independent LEAK respiration. The WI group is marked green, the HTK group is marked yellow and the HTK + CH4 group is marked blue. Data are presented as means ± SD. * p < 0.05 vs. WI; # p < 0.05 vs. HTK (one-way ANOVA and Tukey’s test). Glutamate (Glut), malate (Mal), Oxidative phosphorylation (Oxphos), succinate (Succ), cytochrome c (Cytc), antimycin A (Ama).