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. 2023 Feb 9;12(2):434. doi: 10.3390/antiox12020434

Figure 5.

Figure 5

The effects of NEFA on the expression of PI3K/AKT pathways in BGCs and the concentration screening of pathway activator SC79. BGCs were treated with or without 1.2 mM NEFA for 24 h. (A–D) The protein and gene expression related to PI3K/AKT pathways in BGCs were determined by qRT−PCR assay and Western blot. (E) The cell activity of BGCs was measured using CCK−8 assay using different concentrations of SC79 for 2 h. (F) The cell activity of BGCs that were pretreated with different SC79 concentrations before NEFA treatment. (G,H) Detection of activation of AKT by 5 mg/mL SC79 with Western blot analysis. The quantification of the relative protein levels (performed using Image J) is shown under the band (protein/β−Tubulin). This experiment was repeated 3 times, and data are expressed as mean ± standard errors of the mean (SEM), * p < 0.05; ** p < 0.01; ns, not significant.