Figure 7.

AKT activator SC79 decreased NEFA−induced apoptosis in BGCs. Experiments were performed after NEFA treatment of BGCs for 24 h with or without SC79 pretreatment for 2 h. (A,B) Flow cytometry detected the apoptosis of BGCs. (C–F) The mRNA levels of Bax, Bcl−2, and Caspase−3 were determined using qRT−PCR. (G,H) The apoptosis−related proteins Bax, Bcl−2, and Caspase−3 were analyzed by Western blot. The quantification of the relative protein levels (performed using Image J) is shown under the band (protein/β−Tubulin). This experiment was repeated 3 times, and data are expressed as mean ± standard errors of the mean (SEM), * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.