TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli K-12 SF8 recA | recA56 | 17 |
| P. stutzeri | ||
| JM375 | Rifr Smrhis+ | 11 |
| JM302 | hisX1 | 11, 37 |
| LO15 | JM302 Rifr Nalr | 16 |
| Tf59 | LO15 pilT::Kmr (pSUP102GmTn5B20) | This study |
| Tf591 | LO15 pilU::lacZGmr | This study |
| Tf700 | LO15 ΔpilTU::Gmr | This study |
| Tf300 | pilAI::Gmr | 16 |
| Tf590 | pilAI::GmrpilT::Kmr | This study |
| Plasmids | ||
| pRSF1010d | pRSF1010 with a 800-bp PstI deletion inactivating the Sur gene | 16 |
| pCOM59 | pKT210 carrying 6.2 kb of chromosomal JM375 DNA | This study |
| pCOM59d | pRSF1010d carrying a 3.44-kb PstI fragment of pCOM59 | This study |
| pBluescript II KS(+) | Apr | Stratagene |
| pKS59 | pBluescript II KS(+) carrying a 3.44-kb PstI fragment of pCOM59d | This study |
| pST59 | About 30 kb religated chromosomal DNA of Tf59 including the inserted pSUP102GmTn5B20 | This study |
| pUCP19 | oricolE1 oripRO1600 Apr | 35 |
| pUCPpilT+ | pUCP19 carrying the pilT+ gene of P. stutzeri | This study |
| pAB2001 | AprlacZGmr | 2 |
| pKSpilU+ | pBluescript II KS(+) carrying the pilU+ gene of P. stutzeri | This study |
| pKSpilU::lacZGmr | pKS59 carrying a lacZGmr cassette in the NcoI site of pilU | This study |
| pKS59::Gmr | pKS59 carrying an 1.5-kb BstBI/EcoNI deletion of parts of pilT and pilU; insertion of a Gmr cassette | This study |
| pUCPpilU+ | pUCP19 carrying the pilU+ gene of P. stutzeri | This study |
| pKT240 | Smr oriRSF1010 | 42 |
| pKTpilU | pKT240 carrying the pilU+ gene of P. aeruginosa PAK | 42 |
| pUCPpilT+U+ | pUCP19 carrying the pilT+U+ genes of P. stutzeri | This study |
| pUCPSK | pUCP19-based Pseudomonas-Escherichia shuttle vector with multiple cloning site and promoter sequence of pBluescript II SK(+) | 40 |
| pUCPA1Ha | pUCPSK carrying the pilAI gene with six histidine codons added on the C terminus | This study |
| pUCPA1Hs | pUCPSK carrying the pilAI gene in which the six C-terminal amino acid codons were replaced by six histidine codons | This study |