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. 2001 Aug;183(16):4694–4701. doi: 10.1128/JB.183.16.4694-4701.2001

TABLE 3.

Binding and uptake of [3H]thymidine-labeled chromosomal P. stutzeri DNA by cells of various P. stutzeri strainsa

Strain Relevant genotype Amt (pg) of DNA/5 × 108 cells (n)b
Bound Taken up
LO15 Wild type 144 ± 42 (11) 55 ± 20 (10)
Tf59 pilT 210 ± 36 (3) 20 ± 2 (3)c
Tf591 pilU 135 ± 26 (4) 36 ± 10 (4)
Tf700 pilT pilU 228 ± 59 (5) 14 ± 9 (4)c
Tf591(pKTpilU)d pilU/pilU+Pa 119 ± 33 (6) 43 ± 13 (6)
a

The cells were from the late logarithmic growth phase, in which LO15 cells (wild type) are at the competence maximum (0.5 × 109 to 1 × 109 cells per ml). 

b

n, number of experiments. Data are averages with standard deviations. 

c

The DNA uptake by Tf59 and Tf700 was found by t test to be significantly lower than that of LO15 (P = 0.015 and 0.002, respectively). 

d

The plasmid pKTpilU expresses the pilU+ gene of P. aeruginosa (Pa) (42).