Figure 1.

Dietary Mg supplementation prevented a decrease in renal Klotho expression in rats with metabolic syndrome and 5/6 nephrectomy. Nx rats were fed a normal (0.1%) Mg diet or received a dietary Mg supplementation (0.6%). Sham-operated rats fed a normal P and Mg diet served as controls. (A) Image J software was used to quantify renal Klotho expression obtained by western blotting in all animals from the study (n = 10 animals per group). (B) Western blot bands were cropped showing each experimental group, choosing the animal with the most approximated value to the mean of the group obtained after Image J quantification. Data expressed as mean ± SEM. *** p < 0.001, ** p < 0.01 vs. MetS and ## p < 0.01 vs. MetS+Nx+Mg0.1%.