Figure 2.

Dietary Mg supplementation reduced pro-inflammatory cytokine expression in plasma and aortic tissue induced in rats with metabolic syndrome and 5/6 nephrectomy. Nx rats were fed a normal (0.1%) Mg diet or received a dietary Mg supplementation (0.6%). Sham-operated rats fed a normal P and Mg diet served as controls. (A) Plasmatic levels of Interleukin-6. (B,C) mRNA levels of IL-6 and IL-1β were analyzed by real-time RT-PCR. (D) The p65-NF-κb (p65) protein was determined by western blotting in nuclear extracts. Each lane is representative of each experimental group, and it was included according to the most approximated value to the mean of its group obtained after Image J quantification. (E) Western blot quantification was performed using Image J measuring the integrated optical density and normalized to TFIIB levels. Data expressed as mean ± SEM. *** p < 0.001, ** p < 0.01 vs. MetS and # p < 0.05 vs. MetS+Nx+Mg0.1%.