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General scheme of the ideal circuit in regenerative therapies combining cells with appropriate molecules and biomaterials. (1) Obtaining cells from the patient (for example, from a skin biopsy). (2) In vitro culture and expansion of autologous cells. (3) and (4) cells are seeded on scaffolds along with biomolecules of interest. (5) The scaffold-molecules-cells system is pre-incubated under optimal conditions. (6) The scaffold is implanted in the patient.
