Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 4;12(2):377. doi: 10.3390/antiox12020377

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Changes in iron metabolism in Neuro-2a cells treated with CHIR99021. (A,E) Western blot analysis of FtH and FtL (A), TfR1 and FPN1 (E) protein after the cells were treated with or without 3μM CHIR99021 [29,30] for 72 h. (B,F) Quantification of Western blot data of FtH and FtL (B), TfR1 and FPN1 (F). (C,G,H) qRT-PCR analysis of FtH and FtL (C), TfR1 (G), and FPN1 (H) mRNA levels. (D) ICP-MS detection of total cellular iron levels in Neuro-2a cells treated with or without CHIR99021. The relative expression levels are normalized to β-actin and then expressed as the fold of control group. The data are presented as the mean ± SEM. * p < 0.05, *** p < 0.001, ns, not significant.