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. 2023 Feb 4;12(2):377. doi: 10.3390/antiox12020377

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Comparison of RNA-seq results and verification of the differentially expressed genes between control and CHIR99021-treated cells. (A) Volcano plot of mRNA expression differences. (B) GO enrichment analysis between control and CHIR99021-treated Neuro-2a cells. (C) Statistical classification analysis of KEGG pathways between control and CHIR99021-treated Neuro-2a cells. (D–F) qRT-PCR analysis of Steap1 (D), Bola2 (E) and Kdm6bos (F) mRNA levels with or without CHIR99021 treatment. The mRNA levels are normalized to β-actin mRNA levels and then expressed as the fold of control group. The data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01.